Histone H4 Antibody 2592S from Cell Signaling Technology

Product Specs
ItemHistone H4 Antibody
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number2592S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk, Dm, Z, Sc
TargetH4
Molecular Weight11
HostRabbit
NCBI Full Gene NameH4 clustered histone 1
NCBI Gene AliasesH4-16, H4C11, H4C12, H4C13, H4C14, H4C15, H4C16, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4FA, HIST1H4A
ClonalityPolyclonal
Research AreaChromatin Regulation / Nuclear Function

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Protective Effects of Relaxin 2 (RLXH2) against Hypoxia-Induced Oxidative Damage and Cell Death via Activation of The Nrf2/HO-1 Signalling Pathway in Gastric Cancer CellsCell JSeptember 9, 2023Liguo Wang, Yi Zhou, Hui Lin, Kezhu Hou(Abcam, #ab8227, 1:1000), anti-GAPDH (Sigma-Aldrich, #G8795, 1:1000), and anti-histone 4 (H4) (CST, #2592, 1:1000). The membranes were rinsed with PBS and further incubated with fluorescent-labelled secondary

(2) Heterocycle-containing tranylcypromine derivatives endowed with high anti-LSD1 activityJournal of Enzyme Inhibition and Medicinal ChemistryDecember 31, 2022Rossella Fioravanti et al.loading. H3K4me2, H3K9me2 (Diagenode, Ougrée, Belgium; pAB-035–050, pAb-060–050), and H4 (Cell Signalling #2592) were used according to the manufacturer’s instructions. 2.6.4. Statistical analysis and software tool

(3) CRELD2 is a novel modulator of calcium release and calcineurin-NFAT signalling during osteoclast differentiationScientific reportsAugust 16, 2022Duxfield A, Munkley J, Briggs MD, Dennis EP.antibodies. The following primary antibodies were used: phosphoSMAD2 (#138D4), SMAD2/3 (#D7G7), Histone H4 (#2592) and NFATc1 (#D15F1) from Cell Signalling. Calcineurin A (ab52761) and TGF-β1 (ab215715) from Abcam.

(4) SENP3‐mediated TIP60 deSUMOylation is required for DNA‐PKcs activity and DNA damage repairMedComm (2020)June 1, 2022Yang Han, Xin Huang, Xiaoyu Cao, Yuchen Li, Lei Gao, Jin Jia, Gang Li, Hejiang Guo, Xiaochang Liu, Hongling Zhao, et al.(H9658, Sigma), anti‐acetylated‐lysine antibody (9441s, Cell Signaling Technology), anti‐histone H4 (2592) and anti‐CHK2 pT68 (2661) (both Cell Signaling Technology), anti‐H4K16ac (13534s, Cell Signaling Technology),

(5) Distinct Exosomal miRNA Profiles from BALF and Lung Tissue of COPD and IPF PatientsInternational Journal of Molecular SciencesOctober 31, 2021Gagandeep Kaur, Krishna Prahlad Maremanda, Michael Campos, Hitendra S. Chand, Feng Li, Nikhil Hirani, M. A. Haseeb, Dongmei Li, Irfan Rahmanprobed overnight with antibodies for exosomal surface markers. The antibodies included Histone 4(Cat# 2592) (Cell Signaling Technologies, Danvers, MA, USA), CD9 (Cat# ab92726), CD63 (Cat# ab134045) (Abcam, Waltham,

(6) Endothelium-protective, histone-neutralizing properties of the polyanionic agent defibrotideJCI insightSeptember 8, 2021Shi H, Gandhi AA, Smith SA, Wang Q, Chiang D, Yalavarthi S, Ali RA, Liu C, Sule G, Tsou PS, et al.from MilliporeSigma (catalog 10223565001). Anti–histone H4 was from Cell Signaling Technology (catalog 2592). TLR2 inhibitor C29 was from Medchemexpress (catalog HY-100461), and TLR4 inhibitor TAK 242 was from

(7) Extracellular vesicles from HTLV-1 infected cells modulate target cells and viral spreadRetrovirologyFebruary 23, 2021Pinto DO, Al Sharif S, Mensah G, Cowen M, Khatkar P, Erickson J, Branscome H, Lattanze T, DeMarino C, Alem F, et al.(Santa Cruz Biotechnology, Inc., Cat. # SC-10809); α-histone H4 (Cell Signaling Technology, Cat. # 2592); α-histone H1 (GeneTex, Cat. # GTX114462); IL-8 (Santa Cruz Biotechnology, Cat. # SC-376750); IL-6

(8) TGF-β1 is a regulator of the pyruvate dehydrogenase complex in fibroblastsScientific ReportsDecember 1, 2020Edward R. Smith, Timothy D. Hewitson(Cell Signalling Technology: cat# 9715S) or rabbit polyclonal anti-histone H4 (Cell Signalling Technology: cat# 2592S) overnight at 4 °C diluted in blocking buffer. Membranes were then incubated with an appropriate highly

(9) DJ-1 Proteoforms in Breast Cancer Cells: The Escape of Metabolic Epigenetic MisregulationCellsAugust 26, 2020Domenica Scumaci et al.(1:100; ab 71594; Abcam; Cambridge, UK); H3 (1:1000; 9715; Cell Signaling; Danvers, MA, USA); H4 (1:1000; 2592; Cell Signaling; Danvers, MA, USA). Vimentin (5741, Cell signaling, 1:1000).The detection of primary

(10) Synthesis of an Alkynyl Methylglyoxal Probe to Investigate Nonenzymatic Histone GlycationThe Journal of Organic ChemistryFebruary 7, 2020Qingfei Zheng, Igor Maksimovic, Akhil Upad, David Guber, Yael Davidseparated on SDS-PAGE, and analyzed by western blot with anti-H3 (Abcam, ab10799) and anti-H4 (CST, 2592S) antibodies. In Cellulo Deglycation Assays. HEK 293T cells were treated with a gradient of AlkMGO

(11) Histone proteomics reveals novel post-translational modifications in breast cancerAging (Albany NY)December 8, 2019Angela Mena Perri, Valter Agosti, Erika Olivo, Antonio Concolino, MariaTeresa De Angelis, Laura Tammè, Claudia Vincenza Fiumara, Giovanni Cuda, Domenica ScumaciCell signaling 2578), Histone H3 Antibody (1:1000; Cell signaling 9715), Histone H4 (1:1000; Cell signaling 2592). (C) 2D Western Blot. Red ponceau Staining of membranes and image master analysis. For 2D Western blot

(12) LSD1 suppresses invasion, migration and metastasis of luminal breast cancer cells via activation of GATA3 and repression of TRIM37 expressionOncogeneOctober 31, 2019Xin Hu, Dongxi Xiang, Ying Xie, Luwei Tao, Yu Zhang, Yue Jin, Luca Pinello, Youzhong Wan, Guo-Cheng Yuan, Zhe LiPTM Biolabs (Hangzhou, China) #PTM-612; 1:1000, H3: Cell Signaling 9715S; 1:1000, H4: Cell Signaling 2592S; 1:1000, GAPDH: Bioworld (Nanjing, China) AP0063; 1:5000] were detected using HRP-conjugated anti-rabbit

(13) Epigenetic regulation of ferroptosis by H2B monoubiquitination and p53EMBO reportsJuly 1, 2019Wang Y, Yang L, Zhang X, Cui W, Liu Y, Sun QR, He Q, Zhao S, Zhang GA, Wang Y, et al.(#13969), H3K27me3 (#9733), H3K36me3 (#9763), H4K8ac (#2594), H4K20me3 (#5737), H3 (#14269), and H4 (#2592) were purchased from Cell Signaling Technology. The antiH3K56me1 antibody was purchased from Active Motif

(14) The MBNL/CELF Splicing Factors Regulate Cytosolic Sulfotransferase 4A1 Protein Expression during Cell DifferentiationDrug metabolism and disposition: the biological fate of chemicalsMarch 1, 2019Idris M, Butcher NJ, Minchin RF.Cell Signaling Technology, Danvers, MA), anti–a-tubulin (3873; Cell Signaling Technology), anti-histone H4 (2592; Cell Signaling Technology), and anti– FLAG-M2 (A8592; Sigma). Data Analysis. Data were analyzed with

(15) Epigenetic control of influenza virus: role of H3K79 methylation in interferon-induced antiviral responseScientific ReportsDecember 1, 2018Laura Marcos-Villar, Juan Díaz-Colunga, Juan Sandoval, Noelia Zamarreño, Sara Landeras-Bueno, Manel Esteller, Ana Falcón, Amelia NietoAbcam) and H4K20me2 (1:1000; Active Motif). Polyclonal antibodies to H3K79me2 D15E8, H3K4me3 C42D8, H4 #2592 and H3 D1H2 (1:1000) were from Cell Signaling. For VSV detection, a monoclonal antibody mix against

(16) Quantitative SUMO proteomics reveals the modulation of several PML nuclear body associated proteins and an anti-senescence function of UBC9Scientific ReportsDecember 1, 2018Francis P. McManus, Véronique Bourdeau, Mariana Acevedo, Stéphane Lopes-Paciencia, Lian Mignacca, Frédéric Lamoliatte, John W. Rojas Pino, Gerardo Ferbeyre, Pierre ThibaultVerreault; HIS-tag, 1:5000, 631212, Clontech; Histone H3, 1:1000, 9715, Cell Signaling; Histone H4, 1:1000, 2592, Cell Signaling; PML, 1:200, H-238, Santa Cruz; RAS, 1:1000, 3965, Cell Signaling; SP100, 1:300, ab43151,

(17) Interaction of positive coactivator 4 with histone 3.3 protein is essential for transcriptional activation of the luteinizing hormone receptor geneBiochimica et biophysica acta. Gene regulatory mechanismsOctober 1, 2018Zhao P, Kavarthapu R, Anbazhagan R, Liao M, Dufau ML.H3K36-Ac (#11885), H2A (#2778S), Histone H2A-Ac, Histone H2B (#8135S), Histone H2B-Ac, Histone H4 (#2592), Histone H4-Ac -Ser1/Lys5/Lys/Lys12 (#SC377520), and GST (#2625S) were purchase from Cell Signaling Technology

(18) Amount of daily exercise is an essential stimulation to alter the epigenome of skeletal muscle in ratsJournal of Applied PhysiologyOctober 1, 2018Ikumi Ohsawa, Ryotaro Konno, Ryo Masuzawa, Fuminori KawanoCell Signaling Technology), histone 4 tri-methylated at lysine 20 (H4K20me3; 5737, Cell Signaling Technology), or total H4 (2592, Cell Signaling Technology) were used to detect each protein. The antibody-bound protein was detected

(19) MST2 kinase suppresses rDNA transcription in response to DNA damage by phosphorylating nucleolar histone H2BThe EMBO JournalAugust 1, 2018Dafni Eleftheria Pefani, Maria Laura Tognoli, Deniz Pirincci Ercan, Vassilis Gorgoulis, Eric O'NeillH2B (abcam, ab52484), H2A (Abcam, ab18255), H3 (96C10, Cell Signalling, 3638), H4 (Cell Signalling, 2592), γH2AX (JBW301, Millipore, 16‐193), nucleolin (4E2, Abcam, ab13541), lamin A/C (Cell signalling, 4777),

(20) SUMO2 conjugation of PCNA facilitates chromatin remodeling to resolve transcription-replication conflictsNature communicationsJuly 13, 2018Li M, Xu X, Chang CW, Zheng L, Shen B, Liu Y.(A300-122A; 1:2000) and rabbit α-SPT16 (A302-492A; 1000) were from Bethyl Laboratories. Rabbit α-Histone H4 (#2592; 1:1000) was from Cell Signaling. Mouse α-NWSHPQFEK tag (StrepII tag; A01732; 1:3000) was from GeneScript.

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Histone H4 Antibody

Histone H4 Antibody from Cell Signaling Technology

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Cell Signaling Technology

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Histone H4 Antibody from Cell Signaling Technology

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